Product Description
- High throughput, complete bisulfite conversion of DNA directly from blood, tissue, cells, FFPE and LCM-derived samples
- Compatible with small sample inputs – as few as 10 cells or 50 pg of DNA.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. |
|---|---|
| Conversion | > 99.5% |
| Elution Volume | ≥ 25 µl |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells. |
| Processing Time | 4 hours |
| Recovery | > 80% |
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. |
| Supplemental Info |
