Product Description
- High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.
- Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array. |
|---|---|
| Conversion | >99% |
| Elution Volume | ≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers |
| Input | 500 pg - 2 µg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info |
