Product Description
- Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. |
|---|---|
| Conversion | > 99% |
| Elution Volume | ≥ 25 µl |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 500 pg - 2 µg of DNA. |
| Processing Time | 3 hours |
| Recovery | > 70% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info |
