Product Description
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
- Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
Applicable For | For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
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Elution Volume | ≥ 6 µl |
Equipment | Microcentrifuge, vortex, cell disrupter/pulverizer |
Processing Time | ≤ 10 minutes |
Processing Volume | 10-20 mg (wet weight) fungi or bacteria, 2x108 bacterial cells, or 2x107 yeast cells |
Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
Sample Source | Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
Size Range | Total RNA ≥ 17 nt |
Type | Total RNA |
Yield | Microprep 10 µg RNA (binding capacity) ≥ 6 µl (elution volume) |