Product Description
- Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
- ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Elution Volume | ≥ 30 µl for shallow well, ≥ 10 µl for deep well |
Equipment | Centrifuge with microplate carriers |
Purity | A260/A280 > 1.8 |
Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
Size Range | 75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well |
Yield | ≤ 5 µg total DNA can be recovered. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |