Product Description
- Perform plasmid midipreps in only 16 minutes using a simple spin-column protocol.
- Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
- Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.
DESCRIPTION
TECHNICAL SPECIFICATIONS
Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
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Binding Capacity | 1.2 mg |
Culture Input | ≤ 50 ml |
Elution Volume | ≥ 150 µl |
Endotoxin Levels | ≤ 0.025 EU/µg of Plasmid DNA |
Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge |
Format | Spin-Column |
Processing Time | ≤ 18 min |
Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
Size Range | Up to 200 kb |
Yield | Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 50 ml of overnight culture grown in LB for high copy number plasmids are 200 – 400 µg. |
Supplemental Info |