Product Description
- Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column.
- Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.
- BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
DESCRIPTION
TECHNICAL SPECIFICATIONS
Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
---|---|
Binding Capacity | 100 µg |
Culture Input | ≤ 5 mL |
Elution Volume | 25 µl |
Endotoxin Levels | ≤ 1 EU/µg |
Equipment | Microcentrifuge and/or vacuum manifold |
Format | Spin Column |
Processing Time | 15 min |
Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
Recommended Sample Volume | ≤ 5.0 ml |
Size Range | Up to ~200 kb |
Yield | Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
Supplemental Info |